Organophosphate Hydrolase in Conductometric Biosensor for the Detection of Organophosphate Pesticides
نویسندگان
چکیده
منابع مشابه
Organophosphate Hydrolase in Conductometric Biosensor for the Detection of Organophosphate Pesticides
The research has developed an enzyme biosensor for the detection organophosphate pesticide residues. The biosensor consists of a pair of screen-printed carbon electrode (SPCEs). One of electrodes contains immobilized organophosphate hydrolase (OPH) on a chitosan membrane by cross-linking it with glutaraldehyde. The area of the electrodes was optimized to 3, 5, and 7 mm(2). The OPH was isolated ...
متن کاملOrganophosphorus hydrolase-based assay for organophosphate pesticides
We report a rapid and versatile organophosphorus hydrolase (OPH)-based method for measurement of organophosphates. This assay is based on a substrate-dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently immobilized to the enzyme. This method employs the use of poly(methyl methacrylate) beads to which ...
متن کاملA fluorescence-based biosensor for the detection of organophosphate pesticides and chemical warfare agents
Recently elevated concerns of environmental contamination with organophosphate (OP) insecticides and the possible use of OP nerve agents by terrorists have spurred interest in developing more accurate and sensitive methods for field detection and discrimination of these neurotoxic agents. The analysis of OP neurotoxins typically requires sophisticated equipment, extensive sample preparation tha...
متن کاملSpectroscopic Methods for the Detection of Organophosphate Pesticides –A Preview
Organophosphate pesticides are the ester forms of phosphoric acid usually considered as secure for agriculture uses due to their relatively fast degradation rates. Organophosphorus pesticides have been extensively used in the area of agriculture to manage insect or pests of a number of economically important crops. Organophosphate pesticides are well-known as the inhibitor of acetylcholinestera...
متن کاملDetermination of concentration of organophosphate pesticides
Pesticides are used extensively in agricultural lands in Northern provinces of Iran which makes river ecosystems more vulnerable to pesticides pollution. This study was conducted in three river estuaries (Babolrud, Tajanrud and Gorganrud), focusing mainly on the concentration levels of organophosphate pesticides (diazinon, malathion, azinphos-methyl) in the muscle tissue of golden mullet fish, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Chemistry Insights
سال: 2015
ISSN: 1177-3901,1177-3901
DOI: 10.4137/aci.s30656